https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Impact of maternal undernutrition around the time of conception on factors regulating hepatic lipid metabolism and microRNAs in singleton and twin fetuses https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24304 485) (P < 0.01), p-PDPK-1 (Ser⁴¹) (P < 0.05), and PKCζ (P < 0.05) was higher in the PCUN and PIUN groups, and hepatic PDK4 (P < 0.001) and CPT-1 (P < 0.05) protein abundance was also higher in the PIUN twin fetus. We also found that the expression of a number of microRNAs was altered in response to PCUN or PIUN and that there is evidence that these changes may underlie the changes in the protein abundance of key regulators of hepatic fatty acid β-oxidation in the PCUN and PIUN groups. Therefore, embryo number and the timing of maternal undernutrition in early pregnancy have a differential impact on hepatic microRNA expression and on the factors that regulate hepatic fatty acid oxidation and lipid synthesis.]]> Wed 28 Feb 2024 15:22:08 AEDT ]]> Noncoding RNA regulation of dopamine signaling in diseases of the central nervous system https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25334 Wed 11 Apr 2018 15:03:48 AEST ]]> Transcriptional and epigenetic regulation of labour associated inflammatory genes in the amnion https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27458 Wed 11 Apr 2018 12:49:18 AEST ]]> Term myometrium is characterized by increased activating epigenetic modifications at the progesterone receptor-A promoter https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13859 Sat 24 Mar 2018 10:39:45 AEDT ]]> Impact of embryo number and maternal undernutrition around the time of conception on insulin signaling and gluconeogenic factors and microRNAs in the liver of fetal sheep https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20824 Sat 24 Mar 2018 08:05:56 AEDT ]]> Embryo number and periconceptional undernutrition in the sheep have differential effects on adrenal epigenotype, growth, and development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20508 Sat 24 Mar 2018 07:59:05 AEDT ]]> Potential influence of in utero and early neonatal exposures on the later development of endometriosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29162 Sat 24 Mar 2018 07:35:42 AEDT ]]> EZH2 as a mediator of treatment resistance in melanoma https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29370 Sat 24 Mar 2018 07:34:17 AEDT ]]> Genome-scale oscillations in DNA methylation during exit from pluripotency https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35158 in vitro and in vivo. Exit from pluripotency and priming for differentiation into somatic lineages is associated with genome-wide de novo DNA methylation. We show that during this phase, co-expression of enzymes required for DNA methylation turnover, DNMT3s and TETs, promotes cell-to-cell variability in this epigenetic mark. Using a combination of single-cell sequencing and quantitative biophysical modeling, we show that this variability is associated with coherent, genome-scale oscillations in DNA methylation with an amplitude dependent on CpG density. Analysis of parallel single-cell transcriptional and epigenetic profiling provides evidence for oscillatory dynamics both in vitro and in vivo. These observations provide insights into the emergence of epigenetic heterogeneity during early embryo development, indicating that dynamic changes in DNA methylation might influence early cell fate decisions.]]> Fri 21 Jun 2019 15:13:02 AEST ]]>